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The development of cellular models in aquatic toxicology

Date: 1.1.1992-
Code: K6611
Department: Åbo Akademi University / Faculty of Mathematics and Natural Sciences (MNF), Dept. of Biology
Address: BioCity, Artillerigatan 6, FIN-20520 Åbo
Phone +358-2-2154 265
Fax +358-2-2154 748
E-mail bisomaa@abo.fi
Project leader: FD Boris Isomaa, docent, överassistent (1.1.1992-)
Researchers: FK Henrik Lilius (1.3.1992-)
PhD Christina Råbergh (1.1.1992-)
FK Maria Sandbacka (1.1.1994-)
FK Eva Maria Fröjdö (1.8.1995-)
Type of research: 0 (0=Within duty, 1=Ordered research, 2=Co-operation)
- basic research 10 %
- applied research 45 %
- developmental work 45 %
Man months: Totally: 48 months
Contacts: Ingår i det internationella projektet Multicenter evaluation of in vitro cytotoxicity
Keywords: giftiga ämnen, fisk, myrkylliset aineet, kala, cytotoxicitet, in vitro toxikologi, toxicitet, fiskceller, fluorometriska prober, cytotoxicity, in vitro toxicology, toxicity, fish cells, fluorometric probes,

The need of effective cellular model systems for the assessment of the toxicity of chemicals and for the investigation of their toxic mechanism, has been repeatedly stressed.The aim of this project is to develop and evaluate such model systems for the use, in the first hand, in aquatic toxicology.The model cells used are: hepatocytes, gill epithelial cells and neuronal cells isolated from rainbow trout.Hepatocytes and gill epithelial cells are used as both freshly isolated cells and as primary cultures.In the assessment of the toxicity of chemicals the focus is mainly on sublethal effects caused by chemicals.Fluorometric probes reflecting alterations in cellular functions are used to detect toxic effects.To increase the information that can be obtained and thereby optimizing the possibility to get predictive data for higher levels of organization, our aim is to develop model systems in which several functions can be monitored in the same or in parallel measurements.The possibility to use the expression of stress proteins as indicators of early toxic affects, is also explored.

Publication(s)

15.2.1996 / 19.2.1996