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No 26 / 55

Epidemiology and virulence of the bacterium Flexibacter psychrophilus isolated from farmed salmonids

Date: 1.9.1995-1.1.2000
Code: K6618
Department: Åbo Akademi University / Faculty of Mathematics and Natural Sciences (MNF), The Institute of Parasitology
Address: BioCity, Artillerigatan 6, FIN-20520 Åbo
Phone +358-2-2154 302
Fax +358-2-2154 748
E-mail tom.wiklund@abo.fi
Project leader: FD Tom Wiklund, forskare (1.9.1995-)
Researchers: Msc Kaido Kroon (1.2.1996-31.8.1996)
Type of research: 0 (0=Within duty, 1=Ordered research, 2=Co-operation)
Finnish funding organizations: CIMO (Centre for International Mobility), Helsingfors () FIM 32000
Foreign funding organizations: The Scandinavian - Japan Sasakawa foundation, Sverige () SEK 50000
Man months: Totally: 14 months
Partners: PhD Wakabayashi Hisatsugu, Tokyo, Japan
Keywords: bakterier, epidemiologi, fiskodling, bakteerit, epidemiologia, kalanviljely, Flexibacter psychrophilus, överlevnad, transmission, virulens, regnbågslax, Flexibacter psychrophilus, epidemiology, plasmids, virulence, biochemical characteristics,

The aim of the present prokect is to identify transmissions mechanisms of F. psychrophilus and identify a possible virulence factor present on a 3.7 kb palasmid.The project plan is divided into 6 different sub-projects: 1) identify mechanisms for the transmissions of F. psychrophilus from fish to fish and from farm to farm, 2) identify mechanisms which causes the etablishment of "carrier state" of F. psychrophilus in fish, 3) estimate the survival capasity of "normal" and plasmidless constructions of F. psychrophilus in water during different conditions, 4) serologically, and biochemically characterize Finnish strains of F. psychrophilus, 5) construct the restriction map of the approximately 3,7 kb plasmid from F. psychriphilus isolated in different countries, 6) pathogenity of plasmidless constructions of F. psychophilus.The methods to be used for the detection and identification of the patoghen include immunofluoroscence antibody technique, PCR technique, flow cytometric method, plate count method.

Publication(s)

15.2.1996 / 25.9.1996